CEDENNA:

Promotes science among our youngsters.

Members

  • imprimir

Associate researchers

Pedro A. Orihuela Díaz

Phone(s): (56-2) 7181170, (56-2) 6812108 (Fax)
Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología. Universidad de Santiago de Chile.
Avda. Ecuador 3493, Estación Central, Santiago, Chile.

Current Occupation

  • Associate Researcher.

Professional Degree

  • Licenciate in Biological sciences, Universidad Nacional Mayor de San Marcos, Lima-Perú.
  • Doctor in Biological Sciences, Pontificia Universidad católica de Chile.

Research Areas

  • Mechanism of action of hormones.
  •  Embryology.
  •  Reproductive Toxicology.
  •  Signal transduction.

National and International Committees and Honors

  • Rockeffeller Foundation Fellowship-Training Program in Reproductive Biology. Unidad de Reproducción y Desarrollo, Pontificia Universidad Católica de Chile, 1993-1995.
  • Training fellow of the Latinoamerican Program in Human Reproduction, PLACIRH, 1994-1995.
  • Doctoral fellow of Instituto Milenio de Biología Fundamental y Aplicada, 2000-2001.
  • Postdoctoral Fellow of PROGRESAR and /Bill and Melinda Gates Foundation. 2002-2004.

Guieded Graduate Thesis (Since to date)

  • Ramiro Rincón: Biomarkers of 2-metoxiestradiol in the female genital tract. Doctoral Program in Biotechnology, Facultad de Química y Biología, Universidad de Santiago de Chile, In progress.

 Guided Undergraduate Thesis (Since2006)

  • Patricia Rivas: Effects of 2-methoxyestradiol in the female genital tract. licenciate in Biological Sciences, Pontificia Universidad católica de Chile. 2008.
  • Lorena Oróstica: Role of TNF-a y TGF-b in the silencing of the nongenomic pathway of estradiol in the oviduct. Biochemistry, Facultad de Química y Biología, Universidad de Santiago de Chile, 2009.
  • Israel Rojas: Effect of TNF-a on the nongenomic pathway of estradiol in the oviduct, Biochemistry, Facultad de Química y Biología, Universidad de Santiago de Chile, In progress.

 Projects

  • “Control of egg transport by genomic and non-genomic actions of estradiol in the rat oviduct”. FONDECYT 1030315. Co-investigator (2003-2006).
  • “Signaling transduction pathways involved in the nongenomic pathway of estradiol in the oviduct”. PROGRESAR (PRO-PRE 004/2003). Principal researcher (2003-2005).
  • “Cellular and molecular mechanisms activated by mating involved in the silencing of the nongenomic pathway of estradiol in the oviduct” FONDECYT 1080523. Principal researcher (2008-2011).
  • “Cellular and molecular changes in the extracellular matrix during the menstrual cycle and its role in the in vitro infection Fallopian tubes explants with Neisseria gonorroheae” FONDECYT 1090589. Co-investigator (2009-2012).

  Conferences (2006 to date)

  • Orihuela PA, Parada-Bustamante A, Rios M, Llados C, Villalón MJ, Croxatto HB. Estradiol regula AMPc e IP3 diferencialmente en epitelio y músculo liso del oviducto de la rata. XX Reunión Bienal de la Asociación Latinoamericana de Investigadores en Reproducción Humana 2007; Buenos Aires-Argentina.
  • Rios M, Parada-Bustamante A, Orihuela PA, Croxatto HB. Calbindina 9KD participa en la vía genómica por la cual estradiol acelera el transporte oviductal en la rata. XX Reunión Bienal de la Asociación Latinoamericana de Investigadores en Reproducción Humana 2007; Buenos Aires-Argentina.
  • Quezada M, Orihuela PA, Cuello M, Owen GI. Identificación de posibles marcadores de la acción de 2-metoxiestradiol en células cancerosas y normales de endometrio y mama. XX Reunión Bienal de la Asociación Latinoamericana de Investigadores en Reproducción Humana 2007; Buenos Aires-Argentina.
  • Parada-Bustarnante A, Orihuela PA, Rios M, Cuevas C, Ortiz M, Croxatto H. Mating shuts down estradiol nongenomic signaling pathway in the rat oviduct. XL Annual Meeting of the Society for the Study of Reproduction (SSR). 2007; San Antonio, USA.
  • Oróstica ML, Utz DR, Zuñiga LM, Parada-Bustamante A, Velasque LA, Orihuela PA. La señalización de TNF-a y TGF-b está asociada al cambio en el mecanismo de acción de estradiol en el oviducto. XXI Reunión Bienal Asociación Latinoamericana de Investigadores en Reproducción Humana. 2009; Sao Paulo, Brasil.
  • Utz DR, Oróstica ML, Zuñiga LM, Parada-Bustamante A, Orihuela PA. Regulación de la expression de factores de transcripción asociados al promotor de catecol-o-metil transferasa en el oviducto de la rata. XXI Reunión Bienal Asociación Latinoamericana de Investigadores en Reproducción Humana. 2009; Sao Paulo, Brasil.
  • Oróstica ML, Zuñiga LM, Utz DR, Parada-Bustamante A, Croxatto HB, Velasquez LA, Orihuela PA. TNF-a and TGF-b signaling cascades are associated with changes in the mode of action of estradiol in the rat oviduct. 42nd Annual Meeting of the Society for the Study of Reproduction. 2009; Pittsburgh, USA.
  • Zuñiga LM, Orihuela PA, Croxatto HB. Mating modifies the expression of Nfatc3, Nfkb1, Fos and Jun in Endosalpinx: Possible role in silencing of non-genomic pathway of estradiol. 42nd Annual Meeting of the Society for the Study of Reproduction, 18-22-Julio, 2009-Pittsburgh, USA.

Publications

2018
  1. C. Godoy-Guzmán, C. Nuñez, P. Orihuela, A. Campos, V. Carriel
    Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis
    J. Anat. (2018) 233, pp73-85
  2. C. Godoy-Guzmán, J.L. Fuentes, M. Osses, L. Toledo-Ordoñez, P.A. Orihuela
    The Uterine Tube: From Herophilus to Horacio Croxatto
    Int. J. Morphol., 36(2):387-390, 2018

2017
  1. P. Reuquen, E. Guajardo-Correa, M.L.Oróstica, C. Curotto, A. Parada-Bustamante, H. Cardenas, P.A.Orihuela
    Prolactin gene expression in the pituitary of rats subjected to vaginocervical stimulation requires Erk-1/2 signaling
    Reproductive Biology 17 (2017) 357-362
  2. D. Fano, C. Vásquez-Velásquez, C. Gonzales-Castañeda, E. Guajardo-Correa, P.A. Orihuela, G.F. Gonzales
    N-Butanol and Aqueous Fractions of Red Maca Methanolic Extract Exerts Opposite Effects on Androgen and Oestrogens Receptors (Alpha and Beta) in Rats with Testosterone-Induced Benign Prostatic Hyperplasia
    Evidence-Based Complementary and Alternative Medicine Volume 2017, Article ID 9124240, 10 pages

2016
  1. P. Díaz, H. Cardenas, P.A. Orihuela
    Red Maca (Lepidium meyenii) did not affect cell viability despite increased androgen receptor and prostate-specific antigen gene expression in the human prostate cancer cell line LNCaP
    Andrologia 2016; 48: 922–926 | Published Oct

2017
  1. A. Parada-Bustamante, C. Molina, C. Valencia, M. Flórez, M.C. Lardone, F. Argandoña, A. Piottante, M. Ebensperguer, P.A. Orihuela, A. Castro
    Disturbed testicular expression of the estrogen-metabolizing enzymes CYP1A1 and COMT in infertile men with primary spermatogenic failure: possible negative implications on Sertoli cells
    Andrology, 2017, 5, 486-494 | Published May

2016
  1. A. Parada-Bustamante, M.L. Oróstica, P.Reuquen, L.M. Zuñiga, H. Cardenas, P.A. Orihuela
    The role of mating in oviduct biology
    Mol. Reprod. Dev. 83: 875-883, 2016 | Published 25 Oct
  2. C. Valencia, C. Molina, M. Florez, J. Buñay, R.D. Moreno, P.A. Orihuela, A. Castro, A. Parada-Bustamante
    2-hydroxyoestradiol and 2-methoxyoestradiol, two endogenous oestradiol metabolites, induce DNA fragmentation in Sertoli cells
    Andrologia 2016, 48, 1294-1306 | Published Dec

2017
  1. A. León, P. Reuquen, C. Garín, R. Segura, P. Vargas, P. Zapata, P.A. Orihuela
    FTIR and Raman Characterization of TiO2 Nanoparticles Coated with Polyethylene Glycol as Carrier for 2-Methoxyestradiol
    Appl. Sci. 2017, 7(1), 49 | Published 4 Jan

2015
  1. P. Reuquén, M.L. Oróstica, I. Rojas, P. Díaz, A. Parada-Bustamante, P.A. Orihuela
    Estradiol increases IP3 by a nongenomic mechanism in the smooth muscle cells from the rat oviduct
    Reproduction 150 (4) 331-341; Published Oct 2015
  2. A. Parada-Bustamante, C. Valencia, P. Reuquén, P. Díaz, R. Rincón-Rodriguez, P.A. Orihuela.
    Role of 2-methoxyestradiol, an Endogenous Estrogen Metabolite, in Health and Disease
    Mini Rev Med Chem. 2015;15(5):427-38

2013
  1. R. Rincón-Rodriguez, M.L. Oróstica, P. Díaz, P. Reuquén, H. Cárdenas, P. Orihuela
    Changes in the gene expression pattern induced by 2-methoxyestradiol in the mouse uterus
    Endocrine 44 (3), 773-8

2012
  1. M.L. Orostica, L.M. Zuñiga, D. Utz, A. Parada-Bustamante, L. Velásquez, H. Cárdenas, P. Orihuela
    Tumor necrosis factor-alpha is the signal induced by mating to shutdown a 2-methoxyestradiol nongenomic action necessary to accelerate oviductal egg transport in the rat
    Reproduction 44 (doi:10.1530/REP-12-0389)
  2. O. Saavedra Dahm, P. Solar, H. Díaz, A. Mandel, M.E. Casado, M.S Rivera, P. Orihuela, L. Velásquez, H. Cárdenas
    La heterogeneidad del alfabetismo en salud y el consentimiento informado en Chile
    Sociedad Chilena de Psicología Clínica 30, 125-130
  3. P. Diaz, P. Solar, N. Juica, P. Orihuela, H. Cárdenas, M. Christodoulides, R. Vargas, L. Velásquez
    Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle
    Reprod Biol Endocrinol 10 (1), 56
  4. O. Saavedra Dahm, P. Solar, H. Díaz, A. Mandel, M.E. Casado, M.S Rivera, P. Orihuela, L. Velásquez, H. Cardenas
    The case against template informed consent procedures in biomedical research: heterogeneity in health literacy in Chile
    Terapia Psicológica 30, 127-131

2009
  1. P. Orihuela, L. Zúñiga, M. Ríos, A. Parada-Bustamante, W.D. Sierralta, L. Velásquez, H. Croxatto
    Mating changes the subcellular distribution and the functionality of estrogen receptors in the rat oviduct
    Reproductive Biology and Endocrinology. 7: 139-149

2010
  1. A. Parada-Bustamante, P. Orihuela, M. Ríos, C. Cuevas, M. Orósrtica, L. Velásquez, M.J. Villalón, H. Croxatto
    A non-genomic signaling pathway shut down by mating changes the estradiol-induced gene expression profile in the rat oviduct
    Reproduction 139: 631-644
  2. P. Orihuela
    Ulipristal: a progesterone receptor antagonist as an emergency contraceptive
    Experts Reviews in Obstetrics & Gynecology 5: 13-17.
  3. K. García, P. Rubilar, M. Vargas, H. Cárdenas, M. Ríos, P. Orihuela, R. Vargas, J. Fuher, J.E. Heckels, M. Christodoulides, L. Velásquez
    Nitric oxide is not involved in Neisseria gonorrohoeae-induced cellular damage of human Fallopian tubes in vitro
    Biol Res 43: 39-50, 2010; Accepted 28 Jan 2010

2011
  1. M. Ríos, A. Parada-Bustamante, L. Velásquez, H. Croxatto, P. Orihuela
    Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats
    Reproductive Biology and Endocrinology 23, 69

2012
  1. L. Velásquez, K. García, F. Morales, J. E. Heckels, P. Orihuela, P. Rodas, M. Christodoulides and H. Cárdenas
    Neisseria gonorrhoeae Pilus Attenuates Cytokine Response of Human Fallopian Tube Explants
    Journal of Biomedicine and Biotechnology Volume 2012 (2012), Article ID 491298, 7 pages; Accepted 16 October 2011

back

Associated Institutions: